MII Implementierungsleitfaden Kerndatensatz-Modul Molekulargenetischer Befundbericht
2027.0.0-ballot.1 - Germany

MII IG Kerndatensatz-Modul Molekulargenetischer Befundbericht - Downloaded Version 2027.0.0-ballot.1 See the Directory of published versions

PlanDefinition: Agilent SureSelect Human All Exon V7 Protocol

Offizielle URL: https://www.medizininformatik-initiative.de/fhir/ext/modul-molgen/PlanDefinition/mii-exa-molgen-protocol-agilent-sureselect Version: 2027.0.0-ballot.1
Active Stand: 2026-09-13 Maschinenlesbarer Name: AgilentSureSelectV7

Target enrichment protocol for whole exome sequencing covering 35.8 Mb of the human exome

German

Id: mii-exa-molgen-protocol-agilent-sureselect
Url: Agilent SureSelect Human All Exon V7 Kit Protocol
Version: 2027.0.0-ballot.1
Title: Agilent SureSelect Human All Exon V7 Kit Protocol
Type:

system: http://terminology.hl7.org/CodeSystem/plan-definition-type

code: protocol

display: Protocol

Date: 2026-09-13 17:10:50+0000
Publisher: Medizininformatik-Initiative
Description:

Target enrichment protocol for whole exome sequencing covering 35.8 Mb of the human exome
Jurisdiction: DE
Actions:
DNA Fragmentation: Fragment genomic DNA to 150-200bp using Covaris
Library Preparation: End repair, A-tailing, adapter ligation
Hybridization: Hybridize with SureSelect baits for 24 hours at 65°C
Capture: Streptavidin bead capture and washing
Amplification: PCR amplification of captured library